INFLUENCE OF EPSTEIN BARR VIRUS (EBV) EXPRESSION ON CD4+ T CELLS AT THE MICROENVIRONMENT IN PEDIATRIC HODGKIN LYMPHOMA

Introduction: A unique feature of Hodgkin lymphoma (HL) is the presence CD4+ T cells that surround, protect, and promote survival tumor cells. It was described primary EBV infection elicits EBV-specific with cytotoxic proteins. Since in Argentina, a high incidence EBV-associated pediatric cases, our aim to evaluate influence on CD4+T at microenvironment patients. Methods: 42 patients HL were included this study. evaluated by EBERs situ hybridization (ISH) LMP1 immunohistochemistry (IHC). CD4, CD8, Foxp3, Tbet, GrB, IL10, PD1 CD56 markers analyzed IHC. Survival Kaplan Meier analysis. Results: expression HRS observed 78% ISH Mean cell count not statistically different between EBV+ EBV- (p > 0.05, MW test). However, when functional evaluated, exclusively positively correlated GrB+ = 0.008, r 0.473, Spearman), whereas neither Foxp3+ nor Tbet+ showed statistical correlation Spearman). also displayed positive CD56+ cells, as marker NK 0.006, 0.488, while, contrast, cases trend negative demonstrated 0.06, -0.686, Furthermore, did show In addition, 0.001, 0.536; p 0.04, 0.364, respectively) 0.013, 0.444, All those correlations cases. None associated event free median used cut off value long rank Conclusions: This study reflects CD4 context may be involved activity infection, could direct, since GrB marker. proinflammatory respectively, counterbalanced immunoregulatory Keywords: No conflicts interests pertinent abstract.